Obesity Triggers: Sequencing the Genome Versus Sequencing the Environment

Sequencing the Bonobo Genome

The Bonobo Genome Consortium generated DNA sequencing reads representing the genome of a single bonobo individual. The data consisted of almost 270 million fragment sequences generated on FLX machines from 454 Life Sciences. The fragments derived from FLX standard and Titanium chemistries, and from paired and unpaired protocols. The data was assembled at the J. Craig Venter Institute with the o...

Sequencing the Chimpanzee Genome

Sequence of the chimpanzee (Pan troglodytes) genome should be a high priority. The chimpanzee genome sequence will have a major impact on our understanding of human disease, human evolution, and human population genetics. We propose that the first phase of a project to obtain chimpanzee sequence consist of 3-4X whole-genome shotgun coverage, with the sequence obtained from multiple individuals ...

Sequencing the maize genome.

Sequencing of complex genomes can be accomplished by enriching shotgun libraries for genes. In maize, gene-enrichment by copy-number normalization (high C(0)t) and methylation filtration (MF) have been used to generate up to two-fold coverage of the gene-space with less than 1 million sequencing reads. Simulations using sequenced bacterial artificial chromosome (BAC) clones predict that 5x cove...

Sequencing the Human Genome

The Maize Genome Sequencing Project.

On September 20, 2002, the National Science Foundation (NSF) announced the launch of the Maize Genome Sequencing Project. The momentum for this endeavor has been building within the maize (Zea mays) genetics and larger plant science community for several years. Reasons for launching a concerted effort at this time are at least 4-fold. First, advances in DNA sequencing technology now allow faste...